|
KMID : 0383820120730020084
|
|
Tuberculosis and Respiratory Diseases
2012 Volume.73 No. 2 p.84 ~ p.92
|
|
|
Asian Dust Particles Induce TGF-β1 via Reactive Oxygen Species in Bronchial Epithelial Cells
|
|
Kyung Sun-Young
Yoon Jin-Young
Kim Yu-Jin
Lee Sang-Pyo
Park Jung-Woong
Jeong Sung-Hwan
|
|
|
Abstract
|
|
|
Background: Asian dust storms can be transported across eastern Asia. In vitro, Asian dust particle-induced inflammation and enhancement of the allergic reaction have been observed. However, the fibrotic effects of Asian dust particles are not clear. Production of transforming growth factor β1 (TGF-β1) and fibronectin were investigated in the bronchial epithelial cells after exposure to Asian dust particulate matter (AD-PM10).
Materials and Methods: During Asian dust storm periods, air samples were collected. The bronchial epithelial cells were exposed to AD-PM10 with and without the antioxidant, N-acetyl-L-cysteine (NAC). Then TGF-β1 and fibronectin were detected by Western blotting. The reactive oxygen species (ROS) was detected by the measurement of dicholorodihydrofluorescin (DCF), using a FACScan, and visualized by a confocal microscopy.
Results: The expression of TGF-β1, fibronectin and ROS was high after being exposed to AD-PM10, compared to the control. NAC attenuated both TGF-β1 and fibronectin expression in the AD-PM10-exposed the bronchial epithelial cells.
Conclusion: AD-PM10 may have fibrotic potential in the bronchial epithelial cells and the possible mechanism is AD-PM10-induced intracellular ROS.
|
|
|
KEYWORD
|
|
|
Air Pollutants, Reactive Oxygen Species, Transforming Growth Factor β
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
  
|